Radioimmunoassay of 5-Hydroxy-3-indole Acetic Acid
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چکیده
منابع مشابه
Radioimmunoassay of 5-hydroxy-3-indole acetic acid.
A direct radioimmunoassay of the methyl ester of urinary and serum 5-hydroxy-3-indole acetic acid is described. The antiserum, raised in a rabbit against a conjugate of bovine serum albumin with 5-hydroxytryptamine hemisuccinamide, contained two antigenic fractions, one binding N-acyl 5-hydroxytryptamine, and the other binding methyl ester of 5-hydroxy-3-indole acetic acid, and N-acyl 5-hydroxy...
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THE colorinietric test allegedly specific for the malignant careinoid syndrome was recently observed to be strongly positive in 2 patients who did not have this disease. Observation of excessive elevation of urinary 5-hv-droxy-3-inidole acetic (5-HIAA) by the qualitative chemical test of IJdenfriend and co-workers has beeni considered a specific. siunple, rapid, and reliable means of recognizin...
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Science, a weekly journal, Is published each Friday by the American Association for the Advancement of Science at The Business Press, Incorporated, N. Queen St. and McGovern Ave., Lancaster, P'a. Founded In 1880. it has been since 1900 the official publication of the AAAS. Editorial and Advertising Offices, 1515 Massachusetts Avenue, N.W.. Washington 5, D. C. Telephone, EXecutive 80 or 661. Cab...
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We describe a "high-performance" reversed-phase liquid-chromatographic method for determination of indole-3-acetic acid (I) and 5-hydroxyindole-3-acetic acid (II) in human plasma. I is eluted at 1.0 mL/min with a mixture of 1-pentanesulfonic acid (pH 3.1), methanol, and water. It is detected by fluorometry. A mixture of citric acid/sodium phosphate solution (pH 4.8) and methanol, at 1.5 mL/min,...
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A prior study (13) from this laboratory showed that oxidation of exogenously applied indole-3-acetic acid (IAA) to oxindole-3-acetic acid (OxIAA) is the major catabolic pathway for IAA in Zea mays endosperm. In this work, we demonstrate that OxIAA is a naturally occurring compound in shoot and endosperm tissue of Z. mays and that the amount of OxIAA in both shoot and endosperm tissue is approxi...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1987
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1987.25.2.101